tufA [2019-10-01 13:31:55]

elongation factor Tu

Locus: BSU_01130

Isoelectric point: 4.72

Molecular weight: 43.43 kDa

Protein length: 396 aa

Gene length: 1191 bp

Function: translation

Product: elongation factor Tu

Essential: yes

E.C.: 3.6.5.3

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → Translation factors
• Groups of genes → Essential genes
• Groups of genes → Membrane proteins
• Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue
• Groups of genes → Phosphoproteins → Phosphorylation on a Tyr residue
• Groups of genes → Phosphoproteins → Phosphorylation on either a Ser, Thr or Tyr residue

Gene

Coordinates: 132,882 → 134,072

Phenotypes of a mutant

• essential PubMed

The protein

Protein family

• TRAFAC class translation factor GTPase superfamily (according to UniProt)
• Classic translation factor GTPase family (according to UniProt)

Domains

• tr-type G domain (aa 10-205) (according to UniProt)

Modification

• phosphorylated on Thr-384 by PrkC, dephosphorylated by PrpC PubMed, PubMed
• Cys83 is S-bacillithiolated in B. subtilis and other Bacillus species PubMed
• phosphorylated on Arg-265 PubMed
• phosphorylated on several Arg residues PubMed
• phosphorylated during sporulation on Thr-63 by YabT, dephosphorylated by PrpC PubMed
• phosphorylated on Tyr-270 PubMed

Effectors of protein activity

• phosphorylation by YabT results in inhibtion of GTP hydrolysis; as a result, EF-Tu remains stably bound
• to the ribosome, stalling protein translation PubMed

Structure

• 3U6B (from E. coli , 77% identity) PubMed

Localization

• EF-Tu localizes in a helical pattern underneath the cell membrane and colocalizes with MreB PubMed
• membrane PubMed
• cytoplasm (according to UniProt)

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: rplGB-rpsL-rpsG-fusA-tufA
  Description: PubMed

  Regulatory mechanism

  • stringent response: negative regulation, PubMed, in stringent response

  Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed
  • the mRNA is processed between rplGB and rpsL by RNase Y, this requires the YmcA-YlbF-YaaT complex PubMed

Biological materials

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

Expression vectors

• for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP839, available in Stülke lab
• for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP847, available in Stülke lab

References

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